Cell Washing - 1153 Words

Name: Ranessa Dockery I.D #: 27100017 Course: Immunohematology Lab Instructor: Sala Randall Date: January 22, 2013 Lab #: 1 Title: Cell Washing Aim: To demonstrate quality assurance techniques in performing cell washing. Principle: Cell washing is a common procedure performed in the laboratory. This is a series of careful steps taken to wash red blood cells normally three times intermittently with centrifugation and decanting (Harmening 2012). The procedure serves to remove unbound immunoglobulins, Wharton’s jelly (from cord blood), hemolysed cells and also fibrin. The principle of the centrifuge entails centrifugal filtering of components due to centrifugal force applied and also centrifugal sedimentation†¦show more content†¦The water was discarded. * Steps 3 to 6 were repeated twice. * The final pellet was re-suspended until a cherry red color (similar to 2-5% standards) was obtained. Results: It was observed that the more the sample was washed, the clearer the supernatant appeared and the smaller the pellet became. The final pellet was re-suspended until a cherry red color was observed. The colors and volume of the 2% and 5% standards were also observed and compared to that of the sample since the aim was to achieve a 2-5% suspension. The 2% standard was of a paler red than the 5%. The final suspension of the sample was observed to be between the two standards, however, closer to the color of the 5% standard after further comparison while the volume was equal to that of the 5% standard. Discussion: In cell washing 0.85% NaCl was used to wash the cell each time. This solution was made prior to the lab exercise, and not overnight or days ahead, to ensure that the concentration remained the same as required and no evaporation could take place to alter / increase the such, as this would have adverse effects on the cells. This concentration was chosen since it was isotonic to the concentration of the red blood cells. Since both solutions were isotonic no particle movement occurred and the cells were not altered inShow MoreRelated7.06 Immune System Lab Report Essay1191 Words   |  5 Pagesï » ¿Nicole Perry 7.06 Immune System Lab Report Thoroughly washing your hands can be one of the best ways to prevent the spread of pathogens that cause illnesses. Our bodies have several lines of defense to fight infections. It is very important to do what we can do to avoid the pathogens from entering our system in the first place. Our hands pick up microscopic pathogens from objects that were touched by people who may not have washed their hands very well. When we forget to wash our hands or don’tRead MoreMrsa1450 Words   |  6 Pagesthat can prevent the spread of MRSA (Gould 2002). Washing hands is not kid’s stuff – not in the era of MRSA. Once entrenched MRSA can be extremely difficult to treat. 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